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KMID : 0545120080180010135
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 1 p.135 ~ p.137
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Selective Production of Epothilone B by Heterologous Expression of Propionyl-CoA Synthetase in Sorangium cellulosum
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Han Se-Jong
Park Sang-Woo
Kim Byung-Woo
Sim Sang-Jun
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Abstract
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The metabolic engineering of epothilones, as secondary metabolites, was investigated using Sorangium cellulosum to achieve the selective production of epothilone B, a potent anticancer agent. Thus, the propionyl-CoA synthetase gene (prpE) from Ralstonia solanacearum was heterologously expressed in S. cellulosum to increase the production of epothilone B. Propionyl-CoA synthetase converts propionate into propionyl-CoA, a potent precursor of epothilone B. The recombinant S. cellulosum containing the prpE gene exhibited a significant increase in the resolution of epothilones B/A, with an epothilone B to A ratio of 127 to 1, which was 100 times higher than that of the wild-type cells, demonstrating its potential use for the selective production of epothilone B.
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KEYWORD
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Epothilone, heterologous expression, metabolic engineering, propionyl-CoA synthetase, Sorangium cellulosum
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